作者:Zhou, G (Zhou, Gang)^[ 1,2 ] ; Song, W (Song, Wei)^[ 1,2 ] ; Hou, YZ (Hou, Yongzhao)^[ 1 ] ; Li, Q (Li, Qing)^[ 1,3 ] ; Deng, XL (Deng, Xuliang)^[ 4 ] ; Fan, YB(Fan, Yubo)^[ 1,2 ]

This work describes the fabrication and characterization of a biocompatible magnetic hydroxyapatite (HA) using an ultrasound-assisted co-precipitation method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) were used to characterize the structure and chemical composition of the produced samples. The M-H loops of synthesized materials were traced using a vibrating sample magnetometer (VSM) and the biocompatibility was evaluated by cell culture and MTT (3-(4,5-dimethylthiazol- 2-yl) 22,5-diphenyltetrazolium bromide) assay. Furthermore, in vivo histopathological examinations were used to evaluate the potential toxicological effects of Fe3O4-HA composites on kidney of SD rats injected intraperitoneally with Fe3O4-HA particles. The results showed that magnetic iron oxide particles first replace OH ions of HA, which are parallel to the c axis, and then enter the HA crystal lattice which produces changes in the crystal surface of HA. Chemical bond interaction was observed between PO43- groups of HA and iron ions of Fe3O4. The saturation magnetization (MS) of Fe3O4-HA composites was 46.36 emu/g obtained from VSM data. Cell culture and MTT assays indicated that HA could affect the growth and proliferation of HEK-293 cells. This Fe3O4-HA composite produced no negative effects on cell morphology, viability, and proliferation and exhibited remarkable biocompatibility. Moreover, no inflammatory cell infiltration was observed in kidney histopathology slices. Therefore, this study succeeds to develop a Fe3O4-HA composite as a prospective biomagnetic material for future applications. (c) 2013 Wiley Periodicals, Inc.

作者关键词:hydroxyapatite; Fe3O4; composite; magnetic; biocompatibility

KeyWords Plus:IRON-OXIDE NANOPARTICLES; DRUG-DELIVERY; FE3O4 NANOPARTICLES; SILICA SHELL; MG-FERRITE; TI4+ IONS; CELLS;SPHERES; CANCER; NANOCOMPOSITE

通讯作者地址: Fan, YB (通讯作者)

Beihang Univ, Key Lab Biomech & Mech, Minist Educ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China.

地址:

	[ 1 ] Beihang Univ, Key Lab Biomech & Mech, Minist Educ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China
	[ 2 ] Beihang Univ, Res Inst, Shenzhen 518057, Peoples R China
	[ 3 ] Peking Univ, Sch & Hosp Stomatol, Dent Ctr 2, Beijing 100101, Peoples R China
	[ 4 ] Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China

电子邮件地址:yubofan@buaa.edu.cn

WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA

研究方向:Engineering; Materials Science

Web of Science 类别:Engineering, Biomedical; Materials Science, Biomaterials

文献类型:Article

语种:English

入藏号: WOS:000341130400038

PubMed ID: 24339231

ISSN: 1549-3296

电子 ISSN: 1552-4965

• Impact Factor (影响因子): Journal Citation Reports®

IDS 号: AO2FD

Web of Science 核心合集中的 "引用的参考文献": 45

Web of Science 核心合集中的 "被引频次": 0